A quantitative immunoassay for apolipoprotein B in plasma lipoproteins and subcellular fractions of rat liver.

نویسندگان

  • I J Cartwright
  • J A Higgins
چکیده

HDL in the coronary survivors suggests an impairment in the process of reverse cholesterol transport in which this fraction has been demonstrated to be implicated in animal studies [8]. The results of this study therefore suggest that measurements of these HDL subfractions may provide better indicators of coronary risk than that of total plasma cholesterol which is frequently employed for this purpose but was indistinguishable between the two groups in the present study.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Determination of the intracellular distribution and pool sizes of apolipoprotein B in rabbit liver.

We have investigated the intracellular distribution of apolipoprotein B (apo B) in rabbit liver by immunoblotting, radioimmunoassay (r.i.a.) and enzyme-linked immunoassay (e.l.i.s.a.). Apo B100 was detected in total microsomes, rough microsomes, smooth microsomes, trans-enriched Golgi and cis-enriched Golgi and membrane and cisternal-content subfractions prepared from these fractions. There was...

متن کامل

Biosynthesis of plasma lipoproteins. Incorporation of 14C-glucosamine by cells and subcellular fractions of rat liver.

The incorporation of glucosamlneJ4C into plasma lipoproteins has been measured in the intact rat, in liver slices, and in the liver microsome fraction. The labeling of lipoprotein was studied in microsome subfractions, both in viva with glucosammeJ4C and in vitro with UDP-N-acetylglucosamineW. After isolation of lipoproteins with the aid of carrier plasma, labeled lipoproteins were found in smo...

متن کامل

MICROSOME-MEDIATED BENZO[A]PYRENE-DNA BINDING AND INHIBITION BY CYTOSOLIC FRACTIONS FROM LIVER AND SKIN OF ADULT AND WEANLING RATS

Biotransformation of benzo[a]pyrene (BaP) in the presence of microsomal fractions derived from liver and epiderm of adult and weanling rats was examined. The aim of this study was to evaluate the effect of age on the capacity of two organs in transformation of BaP. Subcellular fractions were prepared from skin and liver by ultracentrifugation and were used as the source of BaP metabolizing enzy...

متن کامل

Apolipoproteins of the orotic acid fatty liver: implications for the biogenesis of plasma lipoproteins.

Rats fed orotic acid develop fatty livers characterized by triglyceride-laden, membrane-bounded vesicles designated "liposomes." We have measured the levels of apolipoproteins in isolated liposomes and other subcellular fractions by SDS-polyacrylamide gel electrophoresis, electrotransfer, and immunodecoration. Apolipoproteins Bh, Bl, E, and C appear to cofractionate; for these proteins, the lip...

متن کامل

Plasma very low density lipoproteins contain a single molecule of apolipoprotein B.

Rat and human very low density lipoproteins (VLDL) were fractionated by zonal ultracentrifugation, yielding sharply defined fractions with narrow sedimentation limits. Sedimentation coefficients for the individual fractions were determined at two densities with the analytical ultracentrifuge, and the results were analyzed to yield buoyant densities and molecular weights for the particles in eac...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Biochemical Society transactions

دوره 18 6  شماره 

صفحات  -

تاریخ انتشار 1990